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A. Sample Preparation

Preparing good quality of sample is the crucial step leading to successful of sequencing. We provides Real Biotech Corporation (RBC) extraction kit to yield purity plamid DNA for sanger sequencing. Make sure the DNA is free of contamination such as unsed primer or dNTPs.

Please provide DNA with concentration of 100ng/uL, at least uL/reaction. Extra amount provided is to ensure our laboratory have enough amount for re-sequencing in the case of failed on first reaction.

Do make sure the sample provided the concentration and amount if the sample is within the required range.

You can send us both purified or non-purified PCR product. For purified PCR product, please make sure the unwanted elements is totally removed otherwise it may yield an unusable sequence results.  Thus, data receiving will be delayed as re-send or re-sequencing needed. For non-purified PCR product, we provide FREE PCR/GEL purification for non-purified sample. We do provide Real Biotech Corporation (RBC) DNA/GEL Purification kit at attractive price.

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B. Sample Quantitation

Quality of the template is also one of the most critical factors lead to successful of sequencing. Ensure the sample is provided within concentration required and the sample should free of contaminant and DNA is not degraded. Low concentration of the template sample may highly affect the final sequencing result.

How?

The most preferred method that used to quatify DNA is Gel Electrophoresis

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C. Primer Preparation

If using own synthesized primer, kindly prepare your primer (Concentration 5μM, volume at least 10μl/reaction) in a microcentrifuge tube, label your primer name on the top of the tube and sealed the tube using parafilm. Place it in the provided zip lock bag together with your sample. Click the button below to see MyTACG Universal Primer Offered