Whole genome sequencing

Uncover secrets of genomes

de novo bacterial whole genome sequencing

With the rapid development of high-throughput sequencing technology and bioinformatics, bacterial whole-genome de novo sequencing has become a cost-effective method to explore bacteriology. Through whole genome sequencing, the gene and related regulatory information of the strain to be tested can be obtained, and the molecular biological basis for providing the unique biological characteristics (pathogenic mechanism, symbiotic mechanism, unique metabolic mechanism) of the strain can be obtained; comparative genomic analysis able to provide theoretical guidance for studying functional differences and evolutionary relationships within and between species.

At present, bacterial genome sequencing has been widely used in many fields such as bacterial epidemiology, vaccine development, and microbial evolution. In addition, bacterial whole genome de novo sequencing builds a comprehensive research platform for the mining of subsequent genomic data and functional analysis of important genes.

Our strategy:-

Bacterial draft genome map:- paired end library construction of 400bp insert size, sequenced by Illumina Miseq or Hiseq platform, assemble the sequencing data in de novo pipeline then perform bioinformatics analysis.

Bacterial complete genome map: Pac Bio RS II library construction of 10kb or 20kb insert size, sequenced by Pac Bio RS II platform, assemble the sequencing data in de novo pipeline then perform bioinformatics analysis.

de novo fungal whole genome sequencing

Fungi belong to lower eukaryotes and are widely found in nature. It is estimated that there are about 1.5 million fungi in the world, including many beneficial fungi with important medicinal value and edible value, as well as a large number of pathogenic fungi that can cause animal and plant diseases. Therefore, the application of beneficial fungi; control and prevention of harmful fungi in our daily life is of great significance to human daily productivity and living. The elucidation of the fungal genome will provide an important basis for the analysis of fungal characteristics. With the maturity of high-throughput sequencing technology, the cost of genome sequencing has been greatly reduced, making it possible to rapidly and economically sequence fungal genomes. At present, fungal genomics research has entered a period of rapid growth.

Our strategy:-

Fungal genome survey:- Illumina PE (~400bp) library was constructed and sequenced by Illumina (Miseq/Hiseq) platform and de novo genome assembly was performed to obtain genomic sequence and functional annotation information.

Fungal genome fine map:- Illumina and Pac Bio libraries were constructed, sequenced by Illumina and Pacbio RS II platforms and de novo genome assembly was performed to obtain genomic fine maps and deep bioinformatics data mining.

Fungal genome chromosome level fine map:- De novo assembly was performed using a combination of multiple sequencing platforms (Pac Bio, optical mapping, or other techniques) to obtain chromosome-level genomes.

de novo plant and animal whole genome sequencing

Whole-genome de novo sequencing means that a species can be sequenced without any reference sequence and assembled by bioinformatics analysis to obtain a whole genome sequence map of the species. Since the completion of the sequencing of the model animal nematode genome in 1998, more than 200 animal genomes have been sequenced, ranging from as few as tens of mega base-pairs (P. cynomolgus, 26.2 Mb) to as large as several giga base-pairs (short-tailed possum, 3.4Gb); Since the completion of the genome sequencing of the Arabidopsis thaliana in 2000, more than 100 plants have been sequenced. In plants that have been sequenced, the genome size range is also large, ranging from a few tens of mega base-pairs (P. tricornutum, 27.4 Mb) to as large as tens of giga base-pairs(Lama pine, 23.2 Gb). The completion of the mapping of a species' genome map indicates that the growth, development, evolution, and origin of the species can be studied at the genomic level, thereby promoting and encouraging research on basic biology, molecular breeding, genetic improvement, and so on. The reveal of whole genome also bring great influences to protection of animals and plants and the variety of excellent germplasm resources that are of great significance to nature and human being.

Our strategy:-

Animal and plant genome survey:- Construction of Illumina PE (250bp, 500 bp) library, sequencing by Illumina platform and k-mer analysis, GC-depth distribution analysis and preliminary assembly were performed to assess the genome complexity of the tested species (genomic size, heterozygosity, GC content and repeat sequence), etc. In addition, analysis of SSR marker development can also be performed by genome survey.

Animal and plant genome fine map:- According to the complexity of the genome, it can be divided into simple genomes and complex genomes;

Simple genome: mainly refers to haploid or homozygous diploids with a repeat ratio of less than 50% (hybridity is less than 0.5%), such as most mammals, birds etc.

Complex genome: mainly refers to diploid or polyploid with a repeat ratio of more than 50% and a heterozygosity of more than 0.5%, such as most aquatic animals and insects, forest trees etc.

Illumina, 10X Genomics, Pacbio libraries were constructed, sequenced by Illumina and Pacbio RS II platform. denovo genome assembly and high-quality genomic maps and deep bioinformatics data mining were obtained by combining transcriptome sequencing data, optical map, Hi-C etc.

Advantages working together with MyTACG

Uncover genomes, empowering NGS bioinformatics

Principal equipped with high standard laboratory and high-throughput sequencing technology platform; reliable, short and fast experiment period;

Principals have a variety of high-throughput sequencing platforms such as Illumina HiSeq 2500/4000, MiSeq, Novaseq 6000, Pac Bio RS II, Pac Bio Sequel, Oxford Nanopore etc.

Experienced personnels to provide experimental solutions, solve experimental problems and analyze experimental results according to customer needs;

Principals have professional bioinformatics team and high performance computing facilities to provide personalized bioinformatics analysis services;

Principal able to provide collaborative paper writing and publication.